一个RNA热传感器控制了Listeria monocytogenes中毒性基因的表达
Jörgen Johansson1, Pierre Mandin, Adriana Renzoni
1Unité des Interactions Bacteries-Cellules, Institut Pasteur, 28, rue du Docteur Roux, 75724 Cedex 15, Paris, France.
Cell
|September 17, 2002
概括
在Listeria monocytogenes prfA mRNA未翻译区域 (UTR) 形成一个温度敏感的结构. 这种结构通过调节翻译启动来控制病毒性基因表达,影响细菌入侵.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌病原体的产生
背景情况:
- 李斯特菌的病毒性基因表达取决于温度,最高温度为37°C.
- 这种温度调节主要由转录激活剂PrfA控制.
- PrfA表达本身是由温度调节的.
研究的目的:
- 研究调节prfA表达的转录后机制.
- 阐明prfA之前的未翻译mRNA (UTR) 在温度依赖基因控制中的作用.
- 探索这种温度调节机制的潜在应用.
主要方法:
- 使用化学探测和原生凝电泳,分析prfA mRNA二次结构.
- 在体外翻译试验测试以评估核糖体结合.
- 局部定向突变发生以改变UTR结构和稳定性.
- 在大肠杆菌中使用GFP进行记者基因测试以验证温度调节.
主要成果:
- 在低温下,prfA mRNA UTR形成了一种二次结构,掩盖了核糖体结合部位.
- 破坏这种结构的突变会激活病毒性基因表达和30°C的细菌入侵.
- 该UTR表现出温度依赖的结构切换,使得差异转换.
- 一个记者构造证明了大肠杆菌的温度依赖光,证实了UTR的调节功能.
结论:
- 一种新的转录后热调节机制通过prfA mRNA UTR控制Listeria monocytogenes的毒性.
- 这种机制涉及UTR的温度依赖的结构变化,调节翻译启动.
- 这些发现为控制细菌基因表达提供了潜在的应用.
相关概念视频
Types of RNA
61.3K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
61.3K
Prokaryotic Transcriptional Activators and Repressors
20.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
20.1K
Riboswitches
8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
Transcriptional Regulation: Riboswitches
1.2K
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
1.2K
Translational Regulation
877
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
877
Regulation of Bacterial Virulence
76
Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...
76


