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一个EGFR/Ebi/Sno通路通过在感应痕信号发送期间失活Su(H) /SMRTER抑制来促进三角体表达
Leo Tsuda1, Raghavendra Nagaraj, S Lawrence Zipursky
1Department of Biological Chemistry and Department of Human Genetics, Molecular Biology Institute, Los Angeles, CA 90095, USA.
Cell
|September 17, 2002
概括
果虫眼中的表皮生长因子受体 (EGFR) 激活促进了Delta表达,启动了一种影响细胞分化的序列. 这一过程涉及到特定的蛋白质,并取决于蛋白质酶的活性.
科学领域:
- 发展生物学 发展生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 切口和表皮生长因子受体 (EGFR) 途径对于细胞增殖和分化至关重要.
- 这些通路之间的交叉交谈显著影响细胞反应.
- 了解它们的相互作用是解读发育过程的关键.
研究的目的:
- 阐明了Drosophila眼睛发育中的EGFR和Notch信号连接机制.
- 为了识别由EGFR激活引发的影响下游信号的顺序事件.
主要方法:
- 利用Drosophila melanogaster作为研究发育途径的模型生物.
- 研究了与EGFR和Notch信号传递相关的基因表达和蛋白质相互作用.
- 采用遗传和蛋白酶体抑制试验来了解调节机制.
主要成果:
- 在正在发育的多索菲拉眼中,EGFR的激活导致光受体分化和三角联体表达.
- 由EGFR诱导的Delta随后向邻近的细胞发出信号,促进它们分化为形细胞.
- 蛋白质ebi和草 (sno) 对于EGFR依赖的Delta转录是必不可少的,因为它们通过调节无毛 (Su(H)) 抑制剂的活性来调节.
- 通过EGFR介导的转录性脱压是蛋白酶体依赖的,涉及SMRTER细胞质转位.
结论:
- 一个连续的信号级联链接EGFR和Drosophila眼睛发育中的Notch通路.
- EGFR信号发射启动了一个过程,最终以Notch介导的细胞命运决定.
- 艾比,sno,Su(H) 和SMRTER的相互作用对于调节这种依赖EGFR的转录控制至关重要.
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