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在Arabidopsis中测量季节性时间的分子基础

Marcelo J Yanovsky¹, Steve A Kay

¹The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA.

|September 20, 2002

概括

植物使用CONSTANS (CO) 蛋白来感知一天的长度,将内部时间与光信号整合起来. 这种机制通过调节开花地点T (FT) 表达,精确地控制了Arabidopsis的开花时间.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
时间生物学时间生物学

背景情况:

有机体测量日长 (光周期) 以预测季节变化并调整发展.
光周期学需要将昼夜时间信息与光感受器的光/黑暗信号相结合.
康斯坦斯 (CO) 蛋白质是对日长反应的开花时间的关键调节者.

研究的目的:

为了阐明Arabidopsis中日长整合的分子机制.
为了证明昼夜系统和光信号如何汇聚在CONSTANS (CO) 功能上.
为了确定参与中介光信号到CO的光受体.

主要方法:

在不同的光周期下分析CONSTANS (CO) 基因表达时间.
通过加密染色体2 (cry2) 和植物染色体A (phyA) 调查光感知作用.
评估CO在光线条件下诱导FLOWERING LOCUS T (FT) 表达的情况.

主要成果:

对CO表达时间的精确昼夜控制对于日长区分至关重要.
在长日中,白天的CO表达必须很高,以便诱导开花.
为了使CO激活FT表达,需要通过加密染色体2 (cry2) 或植物染色体A (phyA) 感知光.

结论:

一个外部巧合机制在CO位置整合昼夜节律和光信号.
这种机制解释了Arabidopsis如何感知白天的长度来调节开花时间.
对CO功能的光调制,加上对其mRNA水平的昼夜控制,是光周期主义的分子基础.

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