红葡萄酒中存在的多类植物类植物素Resveratrol增强了内皮氧化合成酶的表达和活性
Thomas Wallerath1, Göran Deckert, Thomas Ternes
1Department of Pharmacology, Johannes Gutenberg University, Mainz, Germany.
Circulation
|September 25, 2002
概括
在葡萄中发现的白醇可以促进内皮氧化合成酶 (eNOS) 表达和人类细胞中氧化 (NO) 的产生. 这种植物性雌激素.
科学领域:
- 心血管科学 心血管科学
- 分子生物学分子生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 雌激素通过促进剂活性和Sp1转录因子结合来提高内皮氧化合成酶 (eNOS) 的调节.
- 葡萄中的植物激素Resveratrol,作为雌激素受体激动剂.
- 这项研究调查了复星对人类内皮细胞中eNOS表达的影响.
研究的目的:
- 为了确定复星是否影响内皮氧化合成酶 (eNOS) 表达.
- 探索复星影响eNOS的机制.
- 评估复星对氧化 (NO) 生产的短期和长期影响.
主要方法:
- 人类静脉内皮细胞 (HUVEC) 和EA.hy 926个细胞被用Resveratrol化.
- 测量了eNOS mRNA和蛋白质表达,促进剂活性和NO生产.
- 电泳运动转移试验 (EMSA) 用于评估转录因子结合.
- 用对抗剂研究了雌激素受体的作用.
主要成果:
- 复星剂量和时间依赖上调的eNOS mRNA (高达2.8倍) 和蛋白质表达.
- 观察到 eNOS 促进剂活性增加,局部到近位 263 bp.
- Ресвератрол稳定了eNOS mRNA,并增强了NO的产生,独立于雌激素受体对抗作用.
- 短期化也增加了生物活性NO的产生.
结论:
- 复星可以刺激eNOS的表达和活动.
- 这种刺激可能有助于复星的心血管保护作用.
- 该机制涉及对eNOS的直接影响,而不仅仅是通过雌激素受体.
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