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Updated: Jul 28, 2026

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
11:16

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Published on: July 11, 2012

两虫可以支持膜酶的活性

Bonnie M Gorzelle1, Amy Kuhn Hoffman, Melvin H Keyes

  • 1Department of Physiology and Biophysics and Cleveland Center for Structural Biology, Case Western Reserve University, Cleveland, OH 44106-4970, USA.

Journal of the American Chemical Society
|September 26, 2002
PubMed
概括

诸如PMAL-B-100之类的Zwitterionic amphipols可以溶解膜蛋白并维持它们的结构. 这种新的安菲波尔支持完全的酶活性,无需脂质或洗剂.

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Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

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Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
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Flippase
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