一种假定的脂质转移蛋白涉及到Arabidopsis中的系统性抵抗信号
Ana M Maldonado1, Peter Doerner, Richard A Dixon
1The Salk Institute, La Jolla, California 92037, USA.
Nature
|September 28, 2002
概括
一个新的基因,DIR1,对于植物系统性获得性耐药性 (SAR) 是必不可少的. 迪尔1-1突变体无法传输SAR的移动信号,这凸显了DIR1在植物防御信号中的作用.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 局部病原体的攻击会诱导植物的系统性获得性耐药性 (SAR).
- 酸对于局部防御和SAR激活至关重要,但不是移动信号.
- 现有研究表明,酸不是SAR的基本移动信号.
研究的目的:
- 通过使用Agrobacterium tumefaciens转移DNA (tDNA) 标记来识别在SAR中受损的Arabidopsis thaliana中的突变.
- 调查鉴定出突变物在植物防御信号传递中的作用.
主要方法:
- 对tDNA标记的Arabidopsis thaliana线的查,以寻找SAR缺乏突变物.
- 突变系的表型分析,包括局部耐药性和与致病相关的基因表达.
- 皮质体排泄物实验评估移动信号.
主要成果:
- 突变的Directive in induced resistance 1-1 (dir1-1) 显示出正常的局部耐药性,但在远处的叶子中缺乏与致病相关的基因表达.
- dir1-1未能在对病毒性寄生虫 Pseudomonas 和 Peronospora parasitica 产生 SAR.
- 皮质体排泄物实验表明dir1-1在产生或传输移动SAR信号方面有缺陷.
结论:
- DIR1基因对于系统性获得性耐药性 (SAR) 所需的移动信号传递至关重要.
- DIR1编码了一个假定的细胞质脂质转移蛋白,这表明它在基于脂质的远距离信号传输中发挥了作用.
- DIR1对于植物组织之间传输防御信号至关重要,以建立SAR.
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