哺乳动物胚胎的刺介导模式需要运送的传送器类功能
Yong Ma1, Alfrun Erkner, Ruoyu Gong
1Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Cell
|October 10, 2002
概括
小鼠的DispA蛋白拯救了果刺的信号传输. 鼠标DispA对于胚胎发育和刺蛋白释放至关重要,这表明它在不同物种中保留了功能.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 发送 (disp) 基因调节了Drosophila.的远程刺 (Hh) 信号传输.
- 刺信号通路对于胚胎发育和模式形成至关重要.
研究的目的:
- 为了研究Drosophiladisp基因在哺乳动物 Hedgehog信号传递中的小鼠同类的功能.
- 阐明mDispA在小鼠早期胚胎发育和Sonic hedgehog (Shh) 信号传输中的作用.
- 为了表征Disp蛋白在刺蛋白释放中的生物化学活性.
主要方法:
- 在Drosophila中使用小鼠同类的功能性救援实验.
- 在早期小鼠胚胎中分析Hh模式活动.
- 生物化学试验测量Shh释放中的Disp蛋白活性.
主要成果:
- 鼠 mDispA 挽救了 Drosophila 遗传基因的功能.
- 在早期小鼠胚胎中,mDispA对于所有检查的Hh模式活动至关重要.
- 缺乏mDispA的纤维细胞显示其他细胞的SH刺激受损.
- 一个生物化学测试证明了Disp蛋白在释放可溶性Hh蛋白中的作用.
结论:
- 鼠mDispA是Drosophila disp的功能同类物,对哺乳动物刺信号传输至关重要.
- 散射蛋白可能通过一种与细菌载体和Patched保持一致的机制促进子蛋白释放.
- 了解Disp功能提供了对发育信号的保存机制的见解.
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