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相关概念视频

Reproductive Cloning01:27

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

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相关实验视频

Updated: May 10, 2026

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: October 1, 2010

实体细胞的核转移转移

I Wilmut1, N Beaujean, P A de Sousa

  • 1Roslin Institute, Roslin, Midlothian EH25 9PS, UK. ian.wilmut@bbsrc.ac.uk

Nature
|October 11, 2002
PubMed
概括
此摘要是机器生成的。

从成年体细胞进行核转移克隆,显示出显著的发育可塑性. 这个过程可以逆转细胞分化,使细胞核能够直接发展到期.

更多相关视频

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
09:52

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning
04:36

A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning

Published on: December 19, 2025

相关实验视频

Last Updated: May 10, 2026

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: October 1, 2010

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
09:52

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning
04:36

A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning

Published on: December 19, 2025

科学领域:

  • 发育生物学是发展生物学.
  • 细胞重新编程的细胞重编程.
  • 生殖技术 生殖技术

背景情况:

  • 体细胞核转移 (SCNT) 是一种创建克隆后代的技术.
  • 成人体细胞通常具有差异化染色质结构.
  • 卵细胞细胞质中具有能够重编程差异化核的因素.

研究的目的:

  • 为了证明成人体细胞核的发育可塑性.
  • 调查扭转规范差异化的表观遗传修饰的潜力.
  • 确认重编程核能够支持长期全面发展的能力.

主要方法:

  • 身体细胞核的核转移到无核卵细胞中.
  • 在体外培养重建的胚胎.
  • 将可行的胚胎转移到代孕母亲中进行妊娠.

主要成果:

  • 克隆胚胎在核转移后成功发育到期.
  • 有证据表明体细胞分化标志物的逆转.
  • 证明卵细胞的重编程能力.

结论:

  • 通过从成体体细胞中转移核进行克隆是可行的.
  • 卵细胞细胞质可以重新编程分化体细胞核.
  • 发育可塑性是核重编程的一个关键特征.