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相关概念视频

Ion Channels01:19

Ion Channels

The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Resting Potential Decay01:15

Resting Potential Decay

The resting membrane potential of a neuron (-70mV) is sustained due to the selective ion permeability of the membrane. At the resting potential, the membrane is slightly permeable to ions like sodium (Na+) and chloride (Cl−) and highly permeable to potassium ions (K+). Differences in the ions' concentration inside the cell compared to the outside are maintained by membrane transport proteins like channels and pumps.
At rest, the K+ is the main ion that moves across the membrane through...
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Resting Potential Decay01:15

Resting Potential Decay

The resting membrane potential of a neuron (-70mV) is sustained due to the selective ion permeability of the membrane. At the resting potential, the membrane is slightly permeable to ions like sodium (Na+) and chloride (Cl−) and highly permeable to potassium ions (K+). Differences in the ions' concentration inside the cell compared to the outside are maintained by membrane transport proteins like channels and pumps.
At rest, the K+ is the main ion that moves across the membrane through...

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相关实验视频

Updated: Jul 1, 2026

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
08:38

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Published on: May 25, 2011

神经营养素引起的脱极化需要通道Na(V) 1.9

Robert Blum1, Karl W Kafitz, Arthur Konnerth

  • 1Institut für Physiologie, Ludwig-Maximilians-Universität München, D-80336 München, Germany.

Nature
|October 18, 2002
PubMed
概括

大脑衍生神经营养因子 (BDNF) 通过Na(V) 1.9通道激发神经元. 这一发现揭示了神经特洛芬如何激活通道的新奇机制,影响大脑功能和突触可塑性.

科学领域:

  • 神经科学是一个神经科学.
  • 分子生物学分子生物学
  • 细胞信号传输 细胞信号传输

背景情况:

  • 神经营养素,包括来自大脑的神经营养因子 (BDNF),对大脑功能至关重要.
  • BDNF和神经热素-4/5可以激发中枢神经系统的神经元,影响突触可塑性.

研究的目的:

  • 为了阐明底层神经特洛芬引起的神经元激发的分子机制.
  • 为了确定负责神经营养素类似发射器的作用的特定离子通道.

主要方法:

  • 使用反意义信使RNA表达的选候选基因.
  • 与各种通道共同表达TrkB受体氨酸激酶.

主要成果:

  • 确定了对四极毒素不敏感的通道Na(V) 1.9作为神经类蛋白引起刺激的关键介质.
  • 证明Na(V) 1.9是神经质蛋白诱导的神经元脱极化的基础.

结论:

  • 建立了神经特洛芬引起的脱极化的分子基础.
  • 揭示了一种由神经质蛋白介导的通道激活的新机制.

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Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels
11:34

Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels

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Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
07:38

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Published on: March 30, 2015

相关实验视频

Last Updated: Jul 1, 2026

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
08:38

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Published on: May 25, 2011

Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels
11:34

Identification of Specific Sensory Neuron Populations for Study of Expressed Ion Channels

Published on: December 24, 2013

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
07:38

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Published on: March 30, 2015