Dec1和Dec2是哺乳动物分子钟的调节者
Sato Honma1, Takeshi Kawamoto, Yumiko Takagi
1Department of Physiology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
Nature
|October 25, 2002
概括
哺乳动物的昼夜节律是由时钟基因调节的. 新的研究确定了Dec1和Dec2作为关键调节剂,形成了影响分子钟的第五个钟基因家族.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 哺乳动物的昼夜节律是由上神核控制的.
- 四个已知的时钟基因家族形成了转录-翻译反循环,用于细胞内节律的产生.
- 钟和BMAL1蛋白驱动PER基因转录,然后被PER和CRY蛋白抑制.
研究的目的:
- 为了研究 Dec1 和 Dec2 在哺乳动物分子钟中的作用.
- 为了确定Dec1和Dec2是否与已知的时钟基因组件相互作用.
- 描述表达模式和调节的Dec1和Dec2在suprachiasmatic核中的特征.
主要方法:
- 研究了Dec1和Dec2对小鼠Per1促进体活性的影响.
- 分析了Dec1/Dec2和CLOCK/BMAL1.1之间的蛋白质-蛋白质相互作用.
- 检查了上核中的Dec1和Dec2的昼夜表达和光诱导的变化.
主要成果:
- Dec1和Dec2抑制了Per1促进体的CLOCK/BMAL1介导的交换活化.
- 抑制通过与BMAL1的直接相互作用和/或对E-box元素的竞争而发生.
- Dec1和Dec2在上核中表现出昼夜表达,在主观白天达到峰值.
- 光脉冲以相位依赖的方式诱导Dec1的表达.
结论:
- Dec1和Dec2在哺乳动物的分子时钟中充当抑制剂.
- 这些蛋白质代表了一个新的第五个钟基因家族.
- Dec1和Dec2是哺乳动物中昼夜节律的完整调节者.
相关概念视频
Biological Clocks and Seasonal Responses
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
Circadian Rhythms and Gene Regulation
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Circadian Rhythms and Gene Regulation
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...


