高强度,低亲和度的多价值物相互作用和Xenopus laevis中的多种子体的阻断
Esther Arranz-Plaza1, Alex S Tracy, Aloysius Siriwardena
1Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, Athens, Georgia 30602, USA.
Journal of the American Chemical Society
|October 31, 2002
概括
莱克XL35在Xenopus卵细胞上广泛与含有银河糖的糖相互作用,形成阻断多种子症所必需的稳定,保护性层. 多价值增强了结合,并确保了一个强大的受精包裹.
科学领域:
- 生殖生物学 生殖生物学
- 分子相互作用 分子相互作用
- 葡萄糖生物学 葡萄糖生物学
背景情况:
- 莱克XL35和果外套蛋白 (JCP) 的相互作用对于阻止Xenopus laevis多种子症至关重要.
- 这种受精包裹形成背后的精确分子机制尚未完全理解.
研究的目的:
- 阐明受精包膜形成的机制.
- 为了研究XL35和JCP衍生的寡糖之间的相互作用.
主要方法:
- 合成JCP衍生的寡糖与人工氨烯臂.
- 制备单价和多价糖衍生物.
- 利用酶相关的莱克测定和表面等离子体共振来研究XL35的相互作用.
主要成果:
- XL35对含有银河糖的糖具有广泛的特异性,结构特征的亲和力调节较小.
- 多价糖聚合物与单价形式相比,其价值校正的亲缘关系增加了10-20倍.
- 相互作用的特点是极长寿命的结合动力学,反映了原生JCP配体.
结论:
- 在XL35-JCP相互作用中的多价值创造了一个稳定,灵活的保护层,不一定是一个紧密结合的复合体.
- 这一稳定的层确保了多种子体的物理阻碍,即使真正的平衡并没有迅速达到.
- 在多价值相互作用中平衡的缓慢实现可能解释了文献上的差异.
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