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泽斯特/ESC复合体的多索菲拉增强剂具有标记染色体Polycomb位点的素H3甲基转移酶活性

Birgit Czermin¹, Raffaella Melfi, Donna McCabe

¹Adolf-Butenandt Institut, University of Munich, Schillerstrasse 44, 80336 Munich, Germany.

|November 1, 2002

概括

增强Zeste (E(Z)) 蛋白质复合体作为基因素甲基转移酶,特别是在氨酸9和27处甲基化基因素H3. 这种表观遗传标记对于基因沉默和Polycomb蛋白结合至关重要.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学
发展生物学发展生物学

背景情况:

多组蛋白调节基因表达.
增强Zeste (E(Z)) 是一个关键的多组蛋白质.
在早期胚胎中,E (Z) 功能与ESC复合.

研究的目的:

为了研究E(Z) /ESC复合体的酶活性.
为了识别由E(Z) 介导的特定的组素修饰.
为了确定歇斯顿甲基化和Polycomb结合之间的关系.

主要方法:

生物化学分析检测甲基转移酶活性.
在E(Z) SET域的位点定向突变发生.
在聚烯染色体上的染色体免疫沉 (ChIP).
在体外甲基化试验.

主要成果:

该E(Z) /ESC复合物具有向素H3素9和素27的甲基转移酶活性.
变异的E(Z) SET域取消了这种甲基转移酶活动.
H3K9的三甲基化与Polycomb结合部位和异性染色素有关.
在体外甲基化素H3特别结合Polycomb蛋白质.

结论:

E(Z) 是一种基因组甲基转移酶,对于Polycomb介导的基因抑制至关重要.
希斯H3氨酸甲基化是招募Polycomb蛋白质的关键机制.
这种表观遗传修饰在组织色素结构方面发挥着作用.

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