在电压门的K(+) 通道中孔隙开放的能量
Ofer Yifrach1, Roderick MacKinnon
1Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, New York, NY 10021, USA.
Cell
|November 1, 2002
概括
摇器质通道的摇器质通道
科学领域:
- 生物物理学的生物物理.
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 在通道中,电压依赖性封锁涉及电压传感器和孔隙打开之间的机械合.
- 了解这种合对于阐明通道功能和调节至关重要.
研究的目的:
- 为了研究Shaker K ((+) 通道中晚期协调的孔隙开放过渡.
- 分析孔隙突变在通道关和能量合中的作用.
主要方法:
- 在Shaker K(+) 通道的孔隙内利用单个和双重突变.
- 在已知的K(+) 通道结构的背景下解释突变效应.
- 在长达15 Å的距离上评估了能量合.
主要成果:
- 在机械学上重要的毛孔区域中发现了对门敏感的突变.
- 在这些突变和遥远的通道元素之间观察到能量合.
- 道孔在封闭状态下本质上更稳定.
结论:
- 电压传感器必须执行正向工作,施加向外侧的力,以打开K (((+) 通道孔.
- 这种力很可能应用在内螺旋束附近,以克服孔隙的闭合状态稳定性.
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相关概念视频
Non-gated Ion Channels
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Resting Potential Decay
The resting membrane potential of a neuron (-70mV) is sustained due to the selective ion permeability of the membrane. At the resting potential, the membrane is slightly permeable to ions like sodium (Na+) and chloride (Cl−) and highly permeable to potassium ions (K+). Differences in the ions' concentration inside the cell compared to the outside are maintained by membrane transport proteins like channels and pumps.
At rest, the K+ is the main ion that moves across the membrane through...
At rest, the K+ is the main ion that moves across the membrane through...
Voltage-gated Ion Channels
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Non-gated Ion Channels
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Voltage-gated Ion Channels
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Resting Potential Decay
The resting membrane potential of a neuron (-70mV) is sustained due to the selective ion permeability of the membrane. At the resting potential, the membrane is slightly permeable to ions like sodium (Na+) and chloride (Cl−) and highly permeable to potassium ions (K+). Differences in the ions' concentration inside the cell compared to the outside are maintained by membrane transport proteins like channels and pumps.
At rest, the K+ is the main ion that moves across the membrane through...
At rest, the K+ is the main ion that moves across the membrane through...
