相关实验视频

Updated: May 7, 2026

Bacterial Delivery of RNAi Effectors: Transkingdom RNAi

Bacterial Delivery of RNAi Effectors: Transkingdom RNAi

Published on: August 19, 2010

胺衍生物直接与信使RNA结合,以调节细菌基因表达

Wade Winkler¹, Ali Nahvi, Ronald R Breaker

¹Department of Molecular, Cellular and Developmental Biology, Yale University, PO Box 208103, New Haven, Connecticut 06520-8103, USA.

|November 1, 2002

概括

大肠杆菌中的信使RNA可以直接结合胺或其衍生物,形成 рибо开关. 这种结合会改变mRNA结构,减少基因表达而不涉及蛋白质.

科学领域:

分子生物学分子生物学
生物化学生物化学
遗传学遗传学是一个

背景情况:

RNA分子具有结构性可塑性,可以实现酶和受体功能,超出蛋白质作用.
基核糖酶表明RNA具有由效应分子调节的复杂调节的能力.
在信使RNAs (mRNAs) 中的代谢物依赖的全性机制被提议用于基因调节.

研究的目的:

为了调查mRNA是否编码大肠杆菌中的胺生物合成酶,可以直接感知并对胺或其衍生物做出反应.
描述这种代谢物感应mRNA系统的结构和调节机制.

主要方法:

在大肠杆菌中研究了mRNA-effector的结合.
在结合胺或胺酸盐时分析了mRNA的结构变化.
评估了这种结合对基因表达和核糖体结合的影响.

主要成果:

胺生物合成酶的mRNA直接结合胺或胺酸盐,没有蛋白质辅因子.
mRNA-effector复合体形成了一个独特的结构,阻断了核糖体结合部位.
这种结构变化导致基因表达的减少,表明了代谢物感应调节机制.

结论:

在大肠杆菌中发现了一种新的核糖开关机制,其中mRNA直接调节基因表达,基于胺代谢物水平.
这种代谢物感应RNA系统突出显示了RNA的调节潜力,独立于蛋白质.
这些发现表明,核糖开关可能代表一种古老的基因调节形式,早于蛋白质进化.

更多相关视频

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

Published on: June 25, 2015

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

相关概念视频

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Transcription Attenuation in Prokaryotes

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Transcriptional Regulation: Riboswitches

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...

Translational Regulation

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...