线性环林B/Cdc2与复制原点的稳定关联,可以防止Endoreduplication的发生
Jérôme Wuarin1, Vicky Buck, Paul Nurse
1Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.
在裂变酵母中,线性环林依赖激酶Cdc13/Cdc2通过起源识别复合体 (ORC) 结合复制起源. 这种结合确保了线粒分裂在DNA复制之前,但不控制S阶段之间的起源许可.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 细胞循环涉及不同的DNA复制 (S阶段) 和细胞分裂的不同阶段.
- 了解DNA复制原始发射的调节对于防止基因组不稳定至关重要.
- 线粒激酶在调节DNA复制中的作用仍然是活跃的研究领域.
研究的目的:
- 调查线性B型环林Cdc13/Cdc2激酶在核分裂酵母中调节DNA复制起源中的作用.
- 为了确定S阶段对中介线粒分裂的依赖性和复制起源的时间许可.
主要方法:
- 在Cdc13/Cdc2与复制起源的体内关联研究.
- 对具有改变原产地识别复合体 (ORC) 组件的裂变酵母菌株的分析.
- 在Cdc13切除后,同步和观察Endoreduplicating细胞.
主要成果:
- 线性Cdc13/Cdc2激酶与依赖起源识别复合体 (ORC) 的过程中的复制起源相关.
- 这种关联是在染色体复制过程中建立的,并持续到G2和早期的线粒分裂.
- 具有减少Cdc13与原点结合的细胞表现出染色体重复.
- 在内置复制细胞中,Cdc13消去允许在正常细胞周期周期性下获得原产地许可.
结论:
- 与ORC结合的线性Cdc13/Cdc2激酶强制执行了S阶段之前的线性分裂的要求.
- 这种酶复合体并不决定连续的S阶段之间的复制起源许可的时间.
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