在手术前给予谷氨酸会诱导热冲击蛋白70的表达,并通过调节氧化合成酶活性来减弱心肺旁路诱导的炎症反应
Yoshitaka Hayashi1, Yoshiki Sawa, Naoto Fukuyama
1Department of Surgery, Osaka University Graduate School of Medicine, Suita, Osaka, Japan. hayashi@surg1.med.osaka-u.ac.jp
Circulation
|November 13, 2002
概括
在手术前使用谷氨酸会诱导热冲击蛋白70 (HSP70),以防止心肺旁路 (CPB) 诱导的炎症. 这种自我保护机制减少了器官损伤和炎症标志物,提供了潜在的治疗策略.
科学领域:
- 生物医学科学 生物医学科学
- 炎症研究 炎症研究
- 手术生理学 手术生理学
背景情况:
- 热冲击蛋白70 (HSP70) 对于控制炎症和减少器官损伤至关重要.
- 预诱导HSP70激活了对炎症侮辱的自我保护系统.
- 谷氨酸被确定为HSP70的诱导剂,这表明其在缓解外科手术期间炎症方面的潜力.
研究的目的:
- 为了研究手术前谷氨酸的有效性,减轻心肺旁路 (CPB) 诱导的炎症反应.
- 探索谷氨酸诱导HSP70在保护CPB相关器官损伤中的作用.
- 阐明谷氨胺的保护作用背后的机制,包括氧化合成酶 (NOS) 的活性.
主要方法:
- 成年雄性Sprague-Dawley大鼠被分为一个用谷氨酸治疗的组 (G) 和一个对照组 (C).
- 组G接受了手术前和CPB启动之前的谷氨酸 (100mg/kg).
- 两组在34°C下接受60分钟的CPB,在手术后3小时收集样本进行分析.
主要成果:
- 谷氨酸的使用显著降低了CPB后的胰岛素中介素-6和胰岛素-8的血水平.
- 心肌和呼吸系统损伤标志物,包括中性粒细胞粘附,在谷氨胺组显著减弱.
- 在谷氨酸组的心脏,肺和肝脏中观察到高HSP70表达,同时保留了内皮构成氧化合成酶 (eNOS) 活性和减弱的可诱导氧化合成酶 (iNOS) 活性.
结论:
- 在手术前使用谷氨酸可有效诱导HSP70的表达,从而对CPB诱导的炎症提供保护.
- 保护机制包括调节氧化合成酶 (NOS) 活动.
- 这表明一种有希望的自我保护策略来控制CPB诱导的炎症反应.
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