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相关概念视频

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

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相关实验视频

Updated: Jul 10, 2026

mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

哺乳动物RNA聚合酶的动力框架 in vivo.

Miroslav Dundr1, Urs Hoffmann-Rohrer, Qiyue Hu

  • 1National Cancer Institute (NCI), National Institutes of Health, Bethesda, MD 20892, USA.

Science (New York, N.Y.)
|November 26, 2002
PubMed
概括

这项研究揭示了哺乳动物RNA聚合酶I (Pol I) 如何在活细胞中的核糖体基因上组装. 聚I组件作为单独的子单元结合在一起,在基因转录过程中低效地形成临时结构.

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.

背景情况:

  • 哺乳动物RNA聚合酶I (Pol I) 对于核糖体RNA合成至关重要.
  • 了解Pol I in vivo的动态组合和功能至关重要.

研究的目的:

  • 分析哺乳动物RNA聚合酶I组合和延长的动力学.
  • 为了研究Pol I在核糖体基因上的活体功能机制.

主要方法:

  • 活细胞的体内显微镜.
  • 图像数据的计算建模.
  • 对招聘和注册频率的分析.

主要成果:

  • 波兰I机械零部件作为独立的子单位被招募.
  • 组装过程通过超稳定的中间体进行.
  • 确定了体内延长时间;组件的整合是低效的.

结论:

  • 建立了哺乳动物Pol I in vivo功能的动力和机械框架.
  • 该研究强调了在Pol I组装过程中低效的组件结合.

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08:14

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

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相关实验视频

Last Updated: Jul 10, 2026

mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
08:14

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

  • 研究结果提供了对核糖体基因转录的动态调节的见解.