在子中的RNA合成 - - 对reovirus聚合酶lambda3的结构研究
Yizhi Tao1, Diane L Farsetta, Max L Nibert
1Howard Hughes Medical Institute, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Cell
|December 5, 2002
概括
重生病毒lambda3聚合酶晶体结构显示出一个独特的"子"设计. 这种结构解释了酶如何转录双链RNA (dsRNA) 基因组,并表明了有效的RNA聚合的模板保留机制.
科学领域:
- 病毒学 病毒学
- 结构生物学 结构生物学
- 分子生物学分子生物学
背景情况:
- 雷奥病毒聚合酶在蛋白质囊中起作用,转录dSRNA基因组.
- 了解病毒聚合酶结构是病毒复制机制的关键.
研究的目的:
- 为了确定reovirus lambda3聚合酶的晶体结构.
- 阐明病毒囊中RNA聚合的机制.
主要方法:
- 在2.5A分辨率的X射线晶体学.
- 对reovirus lambda3聚合酶的结构分析.
主要成果:
- 兰巴达3聚合酶具有指纹-手掌-指核心与周围的细化,形成一个类似子的结构.
- 四个通道通往催化部位,可视化晶体内的RNA聚合.
- 一个5'帽结合点表明一个模板保留机制,促进RNA合成.
结论:
- 再病毒聚合酶的独特结构促进了dsrna基因组的高效转录.
- 识别的模板保留机制对于酶的功能至关重要.
相关概念视频
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The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
00:43Bacterial RNA Polymerase
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In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
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00:43Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
01:26Size and Structure of Viral Genomes
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