一个含有Drosophila Myb的蛋白质复合体在特定位点DNA复制中的作用
Eileen L Beall1, J Robert Manak, Sharleen Zhou
1Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
Nature
|December 20, 2002
概括
草中的Myb蛋白质复合体结合特定的DNA元素,调节DNA复制过程中的基因放大. 这一发现揭示了特定的蛋白质复合体如何控制DNA复制起源.
科学领域:
- 发育生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 基因复制模式在甲基动物的发育过程中至关重要.
- 在Drosophila的chorion loci中的基因放大涉及特定的cis作用DNA元素.
- 原始识别复合体 (Orc) 绑定了诸如ACE3和Ori-beta.等关键元素.
研究的目的:
- 研究Drosophila Myb蛋白家族在DNA复制和基因放大中的作用.
- 为了识别与Drosophila Myb相关的蛋白质及其结合标.
- 阐明在DNA复制中特定地点起源利用的机制.
主要方法:
- 特定站点的DNA结合测试以确定蛋白质-DNA相互作用.
- 在体内使用Drosophila模型进行基因放大分析.
- 染色体免疫沉以将蛋白质定位到胆位置.
- 报告者转基因测定用于评估复制需求.
主要成果:
- 它的同类Drosophila Myb与其他四种蛋白质形成一个复合体.
- 这种复合物特别与调节性DNA元素 (ACE3和Ori-beta) 结合.
- Drosophila Myb对于胆管基因放大至关重要.
- 在cis中,myb结合点对于记者转基因复制是必要的.
- 在体内,Myb和p120蛋白都局部化到chorion loci.
结论:
- 特定的蛋白质复合体,包括Drosophila Myb,直接参与调节DNA复制.
- 这些复合体与复制增强元件结合,并与复制机器合作.
- 这种相互作用在复制过程中促进了特定地点的来源利用.
相关概念视频
The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Single-Strand DNA Binding Proteins
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...


