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生产缺乏α1,3-银酸转移酶的猪

Carol J Phelps1, Chihiro Koike, Todd D Vaught

  • 1PPL Therapeutics Inc., 1700 Kraft Drive, Blacksburg, VA 24060, USA.

Science (New York, N.Y.)
|December 21, 2002
PubMed
概括

科学家们成功地制造了α1,3-银酸转移酶 (alpha1,3GT) 双击的猪. 这些猪缺乏主要的猪对人类的异抗原,为更安全的异种移植铺平了道路.

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...