在 xenopus 左向右发育过程中,PKCgamma 调节了 syndecan-2 的内向外信号传输
Kenneth L Kramer1, Janet E Barnette, H Joseph Yost
1Center for Children, Huntsman Cancer Institute, Department of Oncological Sciences, University of Utah, Salt Lake City 84112, USA.
Cell
|January 1, 2003
概括
蛋白激酶C gamma (PKCgamma) 控制了右外皮细胞中的合成甘-2酸化,这是左右 (LR) 发育的关键步骤. 这种酸化在早期发育过程中对不对称的基因表达和器官定向至关重要.
科学领域:
- 发展生物学 发展生物学
- 细胞信号传递 细胞信号传递
- 分子生物学分子生物学
背景情况:
- 跨膜蛋白质甘氨酸syndecan-2在左右 (LR) 发育模式中发挥着非自主作用.
- 通过SYNDECAN-2调节LR不对称性的精确分子机制,包括基因表达和器官定向,仍然在很大程度上是未知的.
- LR发育是一个复杂的过程,涉及胚胎发育早期的不对称信号.
研究的目的:
- 为了研究蛋白激酶C玛 (PKCgamma) 在左右 (LR) 发育过程中对合成-2 的调节中的作用.
- 阐明合成甘-2酸化影响LR不对称基因表达和器官定向的机制.
- 通过细胞-细胞相互作用介导的LR发展中最早的分子事件的识别.
主要方法:
- 利用动物帽子的扩展剂来研究外皮细胞与迁徙中皮的相互作用.
- 研究了syndecan-2在右和左外皮细胞中的酸化状态.
- 研究了PKCgamma在调解合成甘-2酸化中的作用.
- 评估了酸化和非酸化合成甘-2状态对于正常LR发育的必要性.
主要成果:
- 证明蛋白激酶C玛 (PKCgamma) 调解了syndecan-2的细胞质域的酸化,特别是在右动物帽外皮细胞中.
- 表明化合成甘-2 (在右细胞中) 和非化合成甘-2 (在左细胞中) 对于正常的LR发育至关重要.
- 确立了这种外皮 - 中皮细胞接触和合成-2-酸化发生在胃流的早期,先于单片的出现.
- 得出结论,PKCgamma调节了syndecan-2酸化,影响了对邻近细胞的内外信号传递.
结论:
- 在早期LR发育过程中,PKCgamma是右侧外皮细胞中syndecan-2酸化的关键调节者.
- 由PKCgamma调节的Syndecan-2酸化状态对于建立LR不对称性至关重要.
- 这项研究揭示了一个早期的,细胞接触依赖的机制,涉及LR模式中的syndecan-2和PKCgamma,先于已知的后期事件,如单形成.
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