相关实验视频

Updated: May 5, 2026

Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

Published on: July 29, 2020

通过光学连贯性断层扫描对动脉样硬化斑块中的巨细胞含量进行量化

Guillermo J Tearney¹, Hiroshi Yabushita, Stuart L Houser

¹Wellman Laboratories of Photomedicine, Massachusetts General Hospital and Harvard Medical School, Boston, Mass 02114, USA. tearney@helix.mgh.harvard.edu

|January 8, 2003

概括

光学连贯断层扫描 (OCT) 可以准确地识别动脉样硬化斑块纤维状盖中的巨细胞. 这种成像技术对检测患者易受损伤的斑块充满希望,有助于风险评估.

科学领域:

心血管成像 - 心血管成像
病理学病理学病理学
生物医学工程生物医学工程

背景情况:

纤维状盖的巨介导降解有助于动脉样硬化斑块的不稳定性.
识别斑块中的巨细胞对于评估脆弱性至关重要.
光学连贯断层扫描 (OCT) 提供高分辨率的血管内成像.

研究的目的:

研究OCT在动脉样硬化纤维状盖内识别巨细胞的实用性.
为了将OCT的发现与巨细胞密度的组织学评估相关联.

主要方法:

26个来自尸体解剖的动脉样硬化动脉片段的OCT成像.
使用CD68免疫氧化酶染色方法对帽子巨细胞密度进行形态计量量化.
OCT信号标准偏差与组织学巨细胞和光滑肌肉动蛋白密度的相关性.

主要成果:

纤维帽巨细胞密度的OCT和组织学之间的高正相关性 (r=0.84).
在OCT和组织学之间显著的负相关性 (r=-0.56) 对于光滑肌肉的动蛋白密度.
境外国家和地区的值实现了100%的灵敏度和特异性,用于检测具有10%以上CD68染色的盖子.

结论:

高对比度,高分辨率的OCT有效地量化了纤维帽中的巨细胞.
海外国家的能力表明它适合于纤维帽的表征.
这项技术有可能在临床环境中识别脆弱的动脉样硬化斑块.

更多相关视频

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes

Published on: August 24, 2021

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

Published on: March 12, 2022

相关概念视频

Inflammation

Inflammation

Atherosclerosis I: Introduction

Atherosclerosis I: Introduction

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...