心内流体力量是胚胎心脏发生的重要表观遗传因素
Jay R Hove1, Reinhard W Köster, Arian S Forouhar
1Options of Bioengineering and Aeronautics, Division of Engineering & Applied Science, California Institute of Technology, Pasadena, California 91125, USA. jhove@caltech.edu
Nature
|January 10, 2003
概括
血液流动模式对于心脏发育至关重要. 斑马鱼胚胎中的高切削力导致心脏异常形成,突出显示出血液动力学.
科学领域:
- 发展生物学 发展生物学
- 心血管生理学心血管生理学
- 生物物理学的生物物理.
背景情况:
- 假设血液流动模式会影响心脏形态发生.
- 室内内皮细胞通过改变细胞骨结构和基因表达来响应流动力.
研究的目的:
- 在体内定量分析斑马鱼胚胎发育中的心内流动力.
- 将体外发现的流动诱导的细胞反应与完整的发育中的心脏联系起来.
- 为了研究心内血动力学在胚胎心脏生成中的作用.
主要方法:
- 在斑马鱼胚胎中的心内血液流量的定量体内成像.
- 基于观察到的流动模式来预测流动诱导的力.
- 心脏输入或输出通道的体内封闭,以破坏正常的血液流动.
主要成果:
- 在斑马鱼心脏发育的关键阶段确定了高剪切,旋流.
- 预测的心内流动力明显高于微型系统的预期.
- 流塞导致心脏缺陷,包括异常的第三腔,受损的循环和缺陷的门形成.
结论:
- 心内血动力学,以高剪切力为特征,对正常的胚胎心脏发育至关重要.
- 在体内正常血流模式的破坏会导致与生俱来的类似心脏的缺陷.
- 血动力学力量作为影响心脏发生的关键表观遗传因素.
相关概念视频
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