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分子伴侣Hsp90和Hsp70向线粒体进口受体Tom70输送前蛋白
Jason C Young1, Nicholas J Hoogenraad, F Ulrich Hartl
1Cellular Biochemistry, Max Planck Institute of Biochemistry, D-82152 Martinsried, Germany.
Cell
|January 16, 2003
概括
细胞质伴侣物如Hsp90和Hsp70通过Tom70受体向线粒体输送蛋白质. 这种相互作用对于蛋白质进口到线粒体至关重要,揭示了一个新的向机制.
科学领域:
- 线粒体生物学 线粒体生物学
- 分子细胞生物学分子细胞生物学
- 蛋白质进口机制是什么
背景情况:
- 细胞分裂因子促进蛋白质向线粒体的确切机制在很大程度上尚不清楚.
- 了解这些通路对于细胞功能和疾病研究至关重要.
研究的目的:
- 为了阐明细胞质伴侣在蛋白质进口到线粒体中的作用.
- 为了确定伴侣和线粒体进口受体之间的特定相互作用.
主要方法:
- 研究了细胞质伴侣体 (Hsp90,Hsp70) 和线粒体导入受体Tom70.0之间的相互作用.
- 利用哺乳动物和酵母模型研究蛋白质在线粒体外膜上的转位.
- 检查了对蛋白质进口中扰乱司机/Tom70识别的功能后果.
主要成果:
- 哺乳动物细胞利用Hsp90和Hsp70,它们与Tom70的TPR域接,提供取决于Hsp90 ATPase活性的进口前蛋白.
- 在酵母中,只有Hsp70参与其中,其与Tom70的对接对于形成功能前蛋白质复合体至关重要.
- 破坏沙龙-Tom70相互作用显著抑制了特定前蛋白质进入线粒体的进口.
结论:
- 陪伴者是由像Tom70这样的膜结合受体招募的,用于特定的蛋白质向线粒体的事件.
- 这项研究揭示了伴侣介导蛋白质进口到线粒体的新机制,突出了物种特异性差异.
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