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使用RNAi对Caenorhabditis elegans基因组进行系统的功能分析

Ravi S Kamath¹, Andrew G Fraser, Yan Dong

¹Wellcome Trust/Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK.

|January 17, 2003

概括

科学家们使用RNA干扰 (RNAi) 来研究C. elegans中的基因功能,确定了1700多个基因的新角色. 这项研究将DNA序列与生物体的发育和行为联系起来,揭示了染色体上的基因聚类.

科学领域:

遗传学和基因组学遗传学和基因组学
发育生物学发展生物学
分子生物学分子生物学

背景情况:

将生物体的DNA序列与其发育和行为联系起来是一个重大的生物挑战.
以往在酵母中使用向删除的基因功能研究取得了成功,但在动物中缺乏类似的综合分析.

研究的目的:

系统地研究动物模型生物C. elegans中的基因功能.
创建一个全面的资源,以了解DNA序列,染色体位置和基因功能之间的关系.

主要方法:

利用RNA干扰 (RNAi) 抑制了C. elegans中大约86%预测基因的功能.
对大量基因组的突变表型进行了鉴定和特征化.
分析了具有已确定功能的基因的染色体分布和转录资料.

主要成果:

鉴定了1,722个基因的突变表型,大约三分之二没有以前已知的相关表型.
发现具有相似功能的基因聚集在C. elegans染色体上不同的大区域.
观察到这些集群中的基因倾向于共享类似的转录资料.

结论:

这项研究显著扩展了C. elegans中已知的基因功能.
证明了基因功能,染色体位置和转录模式之间的相关性.
生成的数据集和RNAi库作为未来系统生物研究的宝贵资源.

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