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母体血中完整的胎儿细胞:它们真的存在吗?
Farideh Z Bischoff1, Sinuhe Hahn, Kirby L Johnson
1Department of Obstetrics and Gynecology, Baylor College of Medicine, 6550 Fannin Street Suite 885, Houston, TX 77030, USA. bischoff@bcm.tmc.edu
Lancet (London, England)
|January 18, 2003
概括
在母体血液中使用胎儿细胞进行非侵入性产前诊断是具有挑战性的,因为细胞稀有. 从母体血中恢复完整的胎儿细胞的尝试没有成功,这表明临床实用性有限.
科学领域:
- 产科和妇科 产科和妇科
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 非侵入性产前诊断 (NIPD) 对胎儿健康评估具有前景.
- 从母亲的血液中恢复罕见的胎儿细胞是复杂的,限制了灵敏度.
- 以前的报道表明,母体血中的完整胎儿细胞通过简单的丰富.
研究的目的:
- 复制从母体血中分离完整的胎儿细胞的技术.
- 评估在NIPD中使用母体血用于胎儿细胞恢复的可行性.
主要方法:
- 五个独立实验室试图识别完整的男性胎儿细胞.
- 对38名携带男性胎儿的妇女的母体血样本进行了分析.
- 正如之前报道的那样,使用了简单的缩方法.
主要成果:
- 在母体血样本中没有发现完整的男性胎儿细胞.
- 多个实验室的复制尝试未能证实完整的胎儿细胞的存在.
- 亡的胎儿细胞可能有助于胎儿DNA检测,但没有达到完整的细胞恢复.
结论:
- 从母体血中恢复完整的胎儿细胞是困难的,对于NIPD来说在临床上是不切实际的.
- 目前的方法不足以可靠地隔离完整的胎儿细胞用于诊断目的.
- 需要进一步的研究来克服胎儿细胞从母体循环中恢复的挑战.
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