在DNA中形成二烯基基离子,反映DNA动态,时间范围为1微秒至1毫秒
Kiyohiko Kawai1, Kei Miyamoto, Sachiko Tojo
1The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan. kiyohiko@sanken.osaka-u.ac.jp
Journal of the American Chemical Society
|January 23, 2003
概括
双重烯结合的寡度氧核化物 (ODN) 揭示了DNA的动态. 二元基离子 (Py2(.+)) 的形成速率通过电荷共振相互作用提供了对短暂DNA结构的洞察.
科学领域:
- 生物物理化学 生物物理化学
- 分子生物学分子生物学
- 频谱学是一种光谱学.
背景情况:
- 与烯 (Py) 结合的氧核化物 (ODN) 具有独特的光谱特性.
- 了解DNA动态对于各种生物过程和治疗应用至关重要.
研究的目的:
- 合成双重烯结合的ODN,用于研究DNA动态.
- 使用脉冲放射溶解测量二烯基基离子 (Py2(.+)) 的形成速率.
- 为了将Py2(.+) 形成动力学与短暂DNA结构动力学相关联.
主要方法:
- 合成双重烯结合的寡度氧核化物 (ODN).
- 在D2O中的ODN溶液的脉冲放射溶解与K2S2O8.8.
- 在470nm和1500nm (电荷共振波段) 的Py2{+}) 基离子 (Py{+}) 的光谱监测.
主要成果:
- 在<5微秒内观察到Py的形成.
- 在100微秒左右观察到Py的同时衰变和Py2的形成.
- Py2的形成速度直接反映了DNA动态,通过电荷共振 (CR) 相互作用捕捉相互作用的Py2物种.
结论:
- 具有其特征的近红外CR吸收带的Py2的形成速度是一个有价值的工具.
- 这种方法提供了短暂形成的DNA结构的结构和动态信息.
- 该研究涵盖了从1微秒到1毫秒的时间范围.
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The DNA Helix
Overview
The DNA Helix
Overview
Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The DNA Helix
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
Chromatin Packaging
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
Nucleic Acid Structure
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
DNA Structure
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