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Low-energy Cathodoluminescence for (Oxy)Nitride Phosphors
Published on: November 15, 2016
新型光酸发生器用于光导向的寡核酸合成
Pawel J Serafinowski1, Peter B Garland
1Cancer Research UK Centre for Cancer Therapeutics and Section of Molecular Carcinogenesis, The Institute of Cancer Research, Sutton, Surrey SM2 5NG, UK. p.serafinowski@icr.ac.uk
Journal of the American Chemical Society
|January 23, 2003
概括
通过产生有效的降解保护剂,新型光敏感可以改善光导向的寡核酸合成. 这种方法实现了与传统技术相比的合成产量,克服了以前的局限性.
科学领域:
- 化学合成 化学合成
- 生物技术是生物技术.
- 有机化学 有机化学
背景情况:
- 光导向的寡核酸合成依赖于依赖光的5'-脱保护.
- 现有的方法往往比传统方法产生更低的阶段性合成产量.
- 问题包括在间接光向中不完全降解保护和酸诱导的净化.
研究的目的:
- 开发新型光敏感,以改善光导聚核酸合成.
- 在紫外线照射时产生有效的降低保护剂.
- 为了获得与传统方法相美的合成产量.
主要方法:
- 开发新型光敏感的2 - 二基 Ester.
- 用近紫外线照射的辐射,以产生α-替代的酸 (例如三酸).
- 在改造DNA合成器上的各种寡核酸的光导合成中的应用和与常规合成的比较.
主要成果:
- 这种新型的光敏感在紫外线照射后成功生成三酸.
- 使用这些的光导合成产生了与常规三酸处理相比的相继结果.
- 这种方法克服了不完整的保护和酸诱导的排尿的局限性.
结论:
- 新型光敏 Ester 为光导聚核酸合成提供了有效的替代品.
- 该方法实现了高的阶段性产量,与传统合成相匹配.
- 这一进步提高了光导向DNA合成的效率和可靠性.
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