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相关概念视频

Mismatch Repair01:36

Mismatch Repair

Overview
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Genome Copying Errors02:46

Genome Copying Errors

DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

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相关实验视频

Updated: Jul 11, 2026

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

在大肠杆菌中的DNA损伤诱导的复制叉回归和处理.

Justin Courcelle1, Janet R Donaldson, Kin-Hoe Chow

  • 1Department of Biological Sciences, Box GY, Mississippi State University, Mississippi State, MS 39762, USA. jcourcelle@biology.msstate.edu

Science (New York, N.Y.)
|January 25, 2003
PubMed
概括

DNA损伤阻碍了复制叉,可以逆转,允许修复. 这种分叉回归由大肠杆菌中的RecA和RecF蛋白稳定,对DNA修复和细胞存活至关重要.

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • 阻止复制的DNA病变是遗传不稳定性和细胞死亡的主要原因.
  • 在大肠杆菌中紫外线损伤后的复制恢复取决于ReCA和recF通路蛋白.

研究的目的:

  • 为了研究在遇到DNA损伤后复制叉恢复的机制.
  • 阐明 RecA,RecF,RecQ 和 RecJ 蛋白在这个过程中的作用.

主要方法:

  • 利用二维阿加罗斯凝电泳来分析复制叉的动态 in vivo.
  • 研究了recA,recF,recQ和recJ基因突变对叉子稳定性的影响.

主要成果:

  • 阻断复制的DNA病变会诱导体内复制叉的短暂逆转 (回归).
  • 反向叉中间体通过ReCA和ReCF稳定.
  • 缺少 RecQ-RecJ 酶-核酶会导致反向叉中间体的降解.

结论:

  • 复制叉回归是处理阻碍DNA合成的DNA病变的关键机制.
  • 这一过程由RecA和RecF等蛋白质介导,允许DNA修复酶进入.

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Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
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Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

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  • 成功的修复能够恢复过程复制,确保细胞活力.