新型光剂用于单分子成像
Katherine A Willets1, Oksana Ostroverkhova, Meng He
1Department of Chemistry, Stanford University, Stanford, California 94305, USA.
Journal of the American Chemical Society
|January 30, 2003
概括
新的非线性光学染色体提供稳定,灵敏的单分子检测. 这些先进的光体可以在广泛的排放范围内调节,并响应当地的环境变化.
科学领域:
- 有机化学 有机化学
- 材料科学 材料科学 材料科学
- 频谱学是一种光谱学.
背景情况:
- 非线性光学 (NLO) 染色体对于先进的光学应用至关重要.
- 单分子检测 (SMD) 需要具有高光量子产量和稳定的光体.
- 与氨基捐赠体配对的二二水受体代表了一个有前途的NLO染色体类别.
研究的目的:
- 为单分子检测提供一类新的NLO染色体.
- 为了证明这些光体的可调节的发射范围和独特特性.
- 探索它们对新型单分子记者实验的潜力.
主要方法:
- 合成和描述六种基于二二二的新型光体.
- 在聚合物宿主中评估光量子产量和光稳定性.
- 测量光物理性质,包括发射光谱,基态二极极时刻和极化性异构.
主要成果:
- 合成了6种光体,它们的辐射范围从505nm到646nm.
- 这些染色体表现出高光量子产量和稳定性,适合SMD.
- 这些分子对局部刚性表现出敏感性,并具有很大的基态二极极子时刻和极化性异质.
结论:
- 基于二二氨酸的NLO染色体对单分子检测有效.
- 它们的可调节的发射和对当地环境的敏感性使新的报告器应用程序成为可能.
- 这些发现扩大了先进单分子光谱和材料设计的工具包.
相关概念视频
01:05Total Internal Reflection Fluorescence Microscopy
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
01:37Super-resolution Fluorescence Microscopy
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