vrille,Pdp1和dClock在Drosophila昼夜时钟中形成了第二个反循环
Shawn A Cyran1, Anna M Buchsbaum, Karen L Reddy
1Department of Biology, New York University, 100 Washington Square East, New York, NY 10003, USA.
Cell
|February 13, 2003
概括
草的昼夜时钟涉及两个反循环. 弗里尔 (VRI) 和等域蛋白1 (PDP1) 调节dClock表达,形成一个关键的第二循环,对于果精确的计时至关重要.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 草的昼夜时钟依赖于相互锁定的转录反循环.
- 一个已知的循环涉及dCLOCK/CYCLE激活周期表达,其中PERIOD蛋白抑制dCLOCK/CYCLE.
- 节奏dClock转录的调节者仍然未被确定.
研究的目的:
- 调查弗里尔 (Vri) 和Pdp1在Drosophila昼夜时钟中的调节作用.
- 阐明VRI和PDP1影响dClock表达的机制.
- 为了确定Pdp1对于昼夜节律的必要性.
主要方法:
- 对dClock表达的VRI和PDP1蛋白反调节的分析.
- 与dClock表达相对的VRI和PDP1水平的时间分析.
- 在Pdp1无突变果中评估昼夜节律性.
主要成果:
- VRI和PDP1蛋白直接调节dClock表达,建立第二个反循环.
- VRI抑制dClock,而PDP1激活它,具有明显的时间峰值 (VRI在PDP1之前3-6小时).
- 节律性Vri转录是分子节律所必需的,而Pdp1被认为是必不可少的时钟基因,因为它缺少会停止时钟.
结论:
- VRI和PDP1与dClock形成了第二个转录反循环,这对于生成节奏dClock表达至关重要.
- 这种VRI-PDP1-dClock循环有助于在Drosophila中产生精确的昼夜节律.
- Pdp1是Drosophila昼夜时钟运作的重要基因.
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