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相关概念视频

Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

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相关实验视频

Updated: Jul 9, 2026

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

Mesd编码了一个LRP5/6的陪伴者,这对于指定小鼠胚胎极性至关重要.

Jen-Chih Hsieh1, Lance Lee, Liqun Zhang

  • 1Department of Biochemistry and Cell Biology, Center for Developmental Genetics, State University of New York, Stony Brook, Stony Brook, NY 11794, USA.

Cell
|February 13, 2003
PubMed
概括
此摘要是机器生成的。

mesd基因对于建立胚胎极性和中皮诱导至关重要,它通过作为WNT信号核心受体的陪伴者来起作用. 它的干扰会损害早期发育,突出其在细胞信号通路中的作用.

更多相关视频

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos
07:53

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos

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Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme
08:36

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Published on: April 12, 2013

相关实验视频

Last Updated: Jul 9, 2026

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos
07:53

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos

Published on: August 3, 2012

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme
08:36

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Published on: April 12, 2013

科学领域:

  • 发展生物学 发展生物学
  • 分子遗传学 分子遗传学
  • 细胞生物学 细胞生物学

背景情况:

  • 胚胎的极性和模式形成依赖于诱导性细胞信号.
  • 研究发育突变有助于理解这些关键的细胞相互作用.

研究的目的:

  • 为了调查mesd基因在胚胎发育中的功能.
  • 阐明MESD在WNT信号传递中的作用及其对极性和中皮诱导的影响.

主要方法:

  • 在小鼠染色体7的中皮发育 (mesd) 删除间隔内的突变分析.
  • 研究MESD在内 плазма网膜中的分子功能.

主要成果:

  • 介质基因对于胚胎极性规范和介质体诱导至关重要.
  • 对于LRP5和LRP6来说,MESD充当了一个特定的陪伴者,这是WNT信号传输的关键组成部分.
  • 缺少mesd的胚胎表现出被破坏的极性和中皮分化,可能是由于WNT信号受损.

结论:

  • 通过其对LRP5/LRP6.6的陪伴活动,MESD对于正规的WNT信号传输至关重要.
  • 虽然WNT信号主要受到影响,但MESD也可能影响其他低密度脂蛋白受体家族成员.
  • 了解MESD的功能,可以了解胚胎发育和细胞信号传递的基本机制.