周围血液的"内皮原生细胞"来自单细胞/巨细胞,分泌血管新生生长因子
Jalees Rehman1, Jingling Li, Christie M Orschell
1Krannert Institute of Cardiology, Department of Medicine, Indiana University School of Medicine, Indianapolis, USA.
Circulation
|March 5, 2003
概括
内皮原生细胞 (EPC) 不增殖,而是分泌血管生长因子,主要通过这些因子而不是直接内皮分化而起作用. 这些发现表明了促进血管生长的新疗法策略.
科学领域:
- 细胞生物学 细胞生物学
- 血管新生研究研究
- 免疫学 免疫学 免疫学
背景情况:
- 来自周围血液的内皮前代细胞 (EPC) 可以增强血管生成.
- 经EPC介导的血管生成 (内皮分化与生长因子分泌) 的机制尚不清楚.
研究的目的:
- 为了研究EPC介导的益血管效应的机制.
- 为了确定EPC是否增殖和分化或分泌血管新生因子.
主要方法:
- 使用乙化LDL吸收和ulex-lectin结合的隔离人类EPC和确认的表型.
- 通过流动细胞计量分析了EPC增殖和表面标记物.
- 对血管生长因子的测试条件条件介质.
主要成果:
- 孤立的EPC主要表达单细胞/巨细胞标记物 (CD14,Mac-1,CD11c),内皮细胞 (VE-cadherin) 或干细胞 (AC133,c-kit) 标记物较少.
- 培养的EPC显示出高调的单细胞激活和巨细胞分化标志物.
- EPCs没有显著的扩散,但分泌了关键的血管原因子 (VEGF,HGF,G-CSF,GM-CSF).
结论:
- 乙-LDL(+) ulex-lectin(+) 细胞 (通常称为EPCs) 主要通过分泌益血管生长因子而起作用,而不是通过增殖或显著的内皮分化.
- 这些细胞主要来自单细胞/巨细胞.
- 血管新生效应很可能由分泌因素介导,这表明基于生长因子的策略或用于治疗缺血的单细胞招募具有治疗潜力.
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