在溶液中的B组脑膜炎球菌多糖的形状灵活性
Terry J Henderson1, Richard M Venable, William Egan
1Edgewood Chemical - Biological Forensic Analytical Center, Battelle Memorial Institute Edgewood Operations, 1204 Technology Drive, Aberdeen, Maryland 21001, USA. txhender@sbcom.apgea.army.mil
Journal of the American Chemical Society
|March 6, 2003
概括
B组脑膜炎球菌多糖体在溶液中表现出显著的灵活性,表现为随机卷. 这表明它的免疫反应并不依赖于一个刚性,有序的形状.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 免疫学 免疫学 免疫学
背景情况:
- 乙组脑膜炎球菌的囊多糖体,α2-->8) 结合的多糖酸,在免疫反应中起着至关重要的作用.
- 了解这种多糖的二次结构和溶液动力学是阐明其免疫性关键.
研究的目的:
- 通过使用NMR光谱学研究alpha(2-->8) 结合的多酸的溶液动力学.
- 要确定二次结构,如螺旋形状,是否会影响对B组脑膜炎球菌的免疫反应.
主要方法:
- 使用核磁共振 (NMR) 光谱,包括 (13) C-[(1) H] NMR 放松时间和稳定状态 NOE 增强.
- 实验性NMR数据使用分子运动模型和不同链长的多糖体 (平均40和400个残留物) 的水力动力学建模来解释.
- 模型考虑了刚性分子的,螺旋形状和内部运动的影响.
主要成果:
- 基于刚性分子翻滚或拟议的螺旋形状的模型无法解释短链或长链长度的NMR测量结果.
- 即使具有广泛的内部运动,螺旋模型也无法再现观察到的NMR数据,这表明这些结构并不占主导地位.
- 核磁放松主要受到内部和细分运动的影响,而不是整体的分子颠倒.
- 对于火环和链接组的相关时间的统计分布表明了显著的灵活性.
结论:
- B组脑膜炎球菌的多糖体存在于溶液中的柔性随机线圈.
- 抗原表位不太可能依赖于这种多糖的刚性,有序的构造.
- 这项研究驳斥了稳定的二次结构在决定对这一关键细菌抗原的免疫反应中的作用.
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