保存的基因素变体H2A.Z保护欧克罗马丁免受无声的异性染色体的异位传播
Marc D Meneghini1, Michelle Wu, Hiten D Madhani
1Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA.
Cell
|March 12, 2003
概括
基斯变体H2A.Z (Htz1) 阻止异色染色素扩散到圣色区域,对抗端粒附近的沉默和交配类型的位置. 删除Htz1允许异色素蛋白扩散,影响基因表达.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 边界元素限制 heterochromatin 的传播,但不能完全解释 euchromatin 的保存.
- 基因组变异H2A.Z (Htz1) 存在于核体中,影响基因表达.
- 端粒和交配类型的位置受沉声机制的调节.
研究的目的:
- 调查H2A.Z (Htz1) 在对抗异性染色素形成和沉默中的作用.
- 了解Htz1在端粒和HMR位置上的功能.
- 阐明Htz1,边界元素和像Sir2和Sir3.3这样的沉默蛋白之间的相互作用.
主要方法:
- 微阵列分析以确定Htz1调节的基因.
- 基因删除研究 (htz1Δ,sir2Δ) 来评估功能相互作用.
- 分析基因组修饰 (乙化,甲基化) 以检测染色质状态的变化.
主要成果:
- 激活HTZ1的基因位于端粒和HMR位点附近.
- 删除Htz1导致这些基因的表达减少,由sir2Δ.救援.
- 在htz1Δ细胞中,Sir2和Sir3扩散到尤克罗马丁中,导致异卵性异卵性染色体的形成.
- Htz1与边界元素协同作用,防止异色色素的传播.
结论:
- H2A.Z (Htz1) 反对端粒和HMR沉默的作用.
- 尤克罗马丁含有诸如Htz1这样的因子,可以防止异性染色质入.
- 异染色素成分也具有对抗欧染色素切换的对抗功能.
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