流体细胞衍生因子-1对ex vivo扩大内皮原生细胞招募缺血性新血管化的影响
Jun-ichi Yamaguchi1, Kengo Fukushima Kusano, Osamu Masuo
1Division of Cardiovascular Research and Medicine, St Elizabeth's Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA.
Circulation
|March 12, 2003
概括
干细胞衍生因子-1 (SDF-1) 增强了内皮原生细胞 (EPC) 的迁移和生存. 局部SDF-1输送促进EPC招募和缺血组织中的新血管化,改善 perfusion.
科学领域:
- 再生医学是一种再生医学.
- 血管生物学 血管生物学
- 干细胞生物学 干细胞生物学
背景情况:
- 流体细胞衍生因子-1 (SDF-1) 是一种化学因子,对造血干细胞贩运至关重要.
- 内皮原生细胞 (EPC) 对于形成新的血管 (血管生成) 至关重要.
- 在血管生成中SDF-1和EPC功能的关系需要进一步研究.
研究的目的:
- 为了研究SDF-1对EPC介导血管生成的影响.
- 为了确定SDF-1是否会增加缺血组织中的EPC招募和新血管化.
主要方法:
- 流细胞计,以评估EPCs上的CXCR4表达.
- 在体外的博伊登室内测定测量EPC向SDF-1的迁移.
- 在体内研究涉及局部SDF-1注射到缺血后肢肌肉裸体小鼠与人类EPC移植.
- 光显微镜用于量化缺血性肌肉中的EPC积累.
- 在治疗后28天评估组织输液和毛细血管密度.
主要成果:
- 这些EPC表达SDF-1受体,CXCR4.
- 根据剂量,SDF-1显著增加了EPC迁移.
- 在实验室中,SDF-1减少了EPC的亡.
- 在体内,局部SDF-1输送增加了缺血肌肉中的EPC积累.
- SDF-1治疗改善了缺血组织输液和增加了毛细血管密度.
结论:
- 当地输送的SDF-1可以增加血管生成.
- SDF-1增强了EPC对缺血组织的招募.
- 通过促进EPC功能,SDF-1有助于缺血性新血管化.
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