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信号传导,转录和粘附之间的联系在上皮细胞芽发育中的发展
Colin Jamora1, Ramanuj DasGupta, Pawel Kocieniewski
1Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, New York 10021, USA.
Nature
|March 21, 2003
概括
两个外部信号,Wnt和BMP抑制剂,通过稳定β-catenin和产生Lef1.1,触发毛囊形成. 这种复合物降低了E-cadherin的调节,使干细胞形态发生和毛囊发育成为可能.
科学领域:
- 发育生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 分子生物学分子生物学
背景情况:
- 机体生成,包括毛囊发育,始于上皮干细胞的衰减.
- 毛囊形态发生涉及干细胞改变极性和细胞-细胞接触以形成芽结构.
研究的目的:
- 阐明在毛囊形态发生的基础上的分子机制.
- 识别发毛囊发育中涉及的信号通路和转录因子.
主要方法:
- 研究了Wnt信号传递和骨形态遗传蛋白 (BMP) 抑制在卵泡形态发生中的作用.
- 在毛囊形成过程中分析了β-catenin,Lef1和E-cadherin之间的相互作用.
- 利用Drosophila模型研究E-cadherin在细胞分裂和细胞骨动态中的功能.
主要成果:
- 同时的Wnt信号和BMP抑制稳定了β-catenin,并产生了Lef1.1.
- 激活的Lef1复合体降低了E-cadherin的调节,这是细胞极性和粘附的关键因素.
- 信号受损或E-cadherin水平升高会扰乱卵泡形态发生和阴道形成.
结论:
- 一个分子程序将Wnt和BMP信号通路与Lef1转录因子形成联系起来.
- Lef1通过降低E-cadherin的调节来重塑细胞结合点,从而促进毛囊的形成.
- 在细胞分裂和粘附中E-cadherin的作用对毛囊发育至关重要.
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