瓦纳氧化酶催化化和烯的循环化
Jayme N Carter-Franklin1, Jon D Parrish, Richard A Tschirret-Guth
1Department of Chemistry and Biochemistry, University of California, Santa Barbara 93106-9510, USA.
Journal of the American Chemical Society
|March 27, 2003
概括
海洋红藻含有化化合物. 来自这些藻类的氧化酶 (V-BrPO) 催化了烯化和循环化,产生了独特的循环,而不是通过简单的反应形成的.
科学领域:
- 海洋天然产品化学 海洋天然产品化学
- 酶学 是一种酶学.
- 有机合成 有机合成
背景情况:
- 海洋红藻 (Rhodophyta) 是生物活性化天然产品的丰富来源,特别是循环烯.
- 这些化合物的生物合成通常归因于海洋氧化酶酶.
- 甲氧化酶 (V-BrPO) 是一种关键酶,涉及海洋环境中有机分子的化.
研究的目的:
- 调查瓦氧化酶 (V-BrPO) 在烯的循环和化中的酶活性.
- 将V-BrPO催化反应的产物与使用水性的非酶反应的产物进行比较.
- 探索V-BrPO在合成复杂的化循环烯中的潜力.
主要方法:
- 从海洋红藻中分离和克隆瓦纳氧化酶 (V-BrPO).
- 在离子和过氧化的存在下,V-BrPO与单二烯 (内醇) 和六二烯 (基拉尼醇) 的酶反应.
- 使用色谱和光谱技术分析反应产品.
- 与在相同条件下使用水性的烯反应进行比较.
主要成果:
- V-BrPO催化了尼罗尔的化和循环化,形成了一个八个组成的循环以太,类似于劳伦辛.
- 维-BrPO催化了化和基拉尼的循环化,产生类似于snyderols的六个成员循环产品.
- 与水性的非酶反应只产生非循环水素,环氧化物和二产物,缺乏循环化.
- 该酶选择性地产生了循环化烯,而化学化产生了非循环产物.
结论:
- 瓦纳氧化酶 (V-BrPO) 有效地指导了烯的电友化和循环化.
- 维BrPO在循环化海洋天然产品的生物合成中发挥着重要作用.
- 这种酶活性为复杂的化循环烯的合成提供了一条新的途径.
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