在水表面的β-hairpin的吸附:一个中子反射研究研究
Jian R Lu1, Shiamalee Perumal, Evan T Powers
1Biological Physics Group, Department of Physics, UMIST, PO Box 88, Manchester M60 1QD, UK. j.lu@umist.ac.uk
Journal of the American Chemical Society
|March 27, 2003
概括
中子反射率显示,β-hairpin单层在空气/水接口上形成均的层. 光素标签对表面覆盖有轻微影响,但对总体β-链形状或表面吸附结构没有影响.
科学领域:
- 表面科学是一门科学.
- 生物物理学的生物物理.
- 材料科学是一种材料科学.
背景情况:
- 贝塔-毛类在蛋白质折叠和功能中至关重要.
- 了解界面上的吸附是生物材料和药物输送的关键.
- 光素标签可以改变的行为,需要仔细的表征.
研究的目的:
- 为了确定空气/水界面上的β-hairpin片单层的厚度和表面覆盖面.
- 研究光标签对的吸附和结构的影响.
- 为了阐明吸附层的构造状态和水合.
主要方法:
- 使用D(2) O对比度的零反射水 (NRW) 进行了中子反射度测量.
- 用D(2) O中的同位素对比度来估计层沉浸的程度.
- 表面过剩和层厚度被分析为酸度的函数.
主要成果:
- 单层14-残留β-hairpin的单层形成均的层,厚度约为10 Å.
- 通过基于BODIPY的光标签,表面覆盖率略有降低 (230 Å2/分子与210 Å2/分子).
- 表面过剩和层厚度随着散装度的下降而减少,表明结构变化.
结论:
- 吸附单层表现出典型的β-链形状的结构特征.
- 发现单层在很大程度上漂浮在水面上,水分含量最小.
- 中子反射性是描述吸附和界面行为的强大工具.
更多相关视频
11:27Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2
Published on: December 8, 2016
10:28Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
Published on: May 27, 2018
相关概念视频
02:35Noncovalent Attractions in Biomolecules
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
01:25Adsorption Isotherms II
Brunauer, Emmett, and Teller (BET) introduced a theory in 1938 that modified Langmuir's assumptions to explain multilayer physical adsorption. This theory is applicable to Type II isotherms and provides a more realistic picture of adsorption processes. The BET theory assumes a uniform solid surface with localized adsorption sites, where adsorption at one site doesn't affect adsorption at neighboring sites. This theory also allows for the possibility of additional molecules being adsorbed on top...