由NADPH氧化酶产生的活性氧物种调节植物细胞生长
Julia Foreman1, Vadim Demidchik, John H F Bothwell
1Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK.
Nature
|March 28, 2003
概括
植物根和根毛发的生长依赖于 (Ca2+) 的吸收,由NADPH氧化酶调节. 这些酶产生活性氧物种 (ROS),激活Ca2+通道,促进细胞扩张和矿物质吸收.
科学领域:
- 植物生物学 植物生物学
- 细胞生理学 细胞生理学
- 分子遗传学 分子遗传学
背景情况:
- 细胞扩张,特别是根和根毛延长,对于植物的水和矿物质吸收至关重要.
- 离子 (Ca2+) 流入对于调节植物根细胞延长速度至关重要.
- 阿拉比多普西斯塔利亚纳rhd2突变体表现出Ca2+吸收受损,导致根毛发缩和细胞扩张受损.
研究的目的:
- 调查控制在生长中的植物根细胞中获得Ca2+的监管机制.
- 确定RHD2基因在Ca2+吸收和根部发育中的作用.
主要方法:
- 描述RHD2基因产物作为NADPH氧化酶的特征.
- 在野生型和rhd2突变根毛中测量活性氧物种 (ROS) 水平.
- 使用二 (DPI) 抑制NADPH氧化酶活性.
- 用ROS对rhd2突变体进行实验性治疗,以观察表型救援.
主要成果:
- RHD2被确定为一种NADPH氧化酶,负责生长根毛中ROS的产生.
- 与野生类型相比,rhd2突变体中的ROS水平显著降低.
- 抑制NADPH氧化酶活性将rhd2突变表型的表拷贝.
- ROS治疗部分挽救了rhd2突变表型,并增强了Ca2+通道活性.
结论:
- NADPH氧化酶通过产生ROS,在植物根的发育中起着至关重要的作用.
- 由NADPH氧化酶产生的ROS通过激活血Ca2+通道来调节植物细胞扩张.
- 这一途径突出了控制Ca2+获取和根形态发生的新机制.
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