移动DNA在抗万科胺素抗性Enterococcus faecalis的进化中的作用
I T Paulsen1, L Banerjei, G S A Myers
1Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. ipaulsen@tigr.org
Science (New York, N.Y.)
|March 29, 2003
概括
抗万科胺素Enterococcus faecalis V583的基因组含有显著的移动DNA,包括一种新的vanB耐药性转位子和独特的等离子体. 这种基因流动性有助于快速抗生素耐药性在肠球菌中传播.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 菌 (Enterococcus faecalis) 是医院感染的常见原因之一.
- 肠球菌中的万科米辛耐药性是一个重大的公共卫生问题.
- 了解抗生素耐药性的遗传基础对于开发新疗法至关重要.
研究的目的:
- 为了进行Enterococcus faecalis V583的完整基因组测序,一种抗万科米辛的临床分离物.
- 识别移动遗传元素及其在抗生素耐药性中的潜在作用.
- 描述V583基因组内的新型耐药机制和质粒.
主要方法:
- 关于Enterococcus faecalis V583.3的全基因组测序,这是一个很好的方法.
- 生物信息分析用于识别移动DNA元素,包括转子子和等离子体.
- 比较基因组学用于识别新型基因和元素.
主要成果:
- 超过25%的E. faecalis V583基因组包括移动或外来DNA.
- 发现了一种新的vanB-vancomycin-resistance结合型转子体.
- 发现了三种等离子体,其中包括两种激素感应的结合性等离子体,其中一种具有新型激素抑制剂.
结论:
- E. faecalis V583的基因组富含移动遗传元素,有助于其对万科素的耐药性.
- 确定了新的移动元素,包括vanB转子和独特的等离子体.
- 移动DNA的高含量可能有助于肠球菌快速获得和传播抗生素耐药性.
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