在血管光滑肌肉中,C-反应蛋白对血管素1型受体进行上调
Chao-Hung Wang1, Shu-Hong Li, Richard D Weisel
1Division of Cardiac Surgery, University of Toronto, Toronto, Ontario, Canada.
Circulation
|April 1, 2003
概括
C-反应蛋白 (CRP) 通过增加血管光滑肌细胞中的血管素1型受体 (AT1-R) 表达来促进动脉样硬化. 洛萨坦是一种AT1-R阻断剂,在体外和体内减弱了这些CRP诱导的动脉样硬化作用.
科学领域:
- 心血管生物学 心血管生物学
- 炎症研究 炎症研究
- 分子医学是分子医学.
背景情况:
- C-反应蛋白 (CRP) 与血管疾病的进展有关.
- 通过激活内皮细胞,CRP可能会积极促进动脉样硬化病变的形成.
- 血管素1型受体 (AT1-R) 在动脉样硬化中起着至关重要的作用.
研究的目的:
- 为了研究CRP对AT1-R表达和动力学在血管光滑肌 (VSM) 细胞的影响.
- 评估CRP对VSM迁移,扩散和活性氧物种 (ROS) 生产的影响,有或没有洛萨坦.
- 为了检查CRP在Neointimal形成中的作用,在鼠 carotid血管成形模型中.
主要方法:
- 研究了人类复合CRP对AT1-RmRNA和培养人类VSM细胞中的蛋白质的影响.
- 在实验室中评估了VSM迁移,扩散和ROS生产.
- 利用大鼠动脉气球损伤模型来研究neointimal形成 in vivo.
主要成果:
- 在VSM细胞中,CRP显著上调AT1-RmRNA,蛋白质表达和细胞表面数.
- CRP增强了VSM的迁移,扩散和ROS的产生,增强了血管新生素II的作用.
- 在体内,CRP增加了neointimal形成,VSM迁移,增殖和AT1-R表达,洛萨坦减少了效应.
结论:
- 血脉光滑肌细胞中的AT1-R介导的动脉样硬化事件在体外和体内都受到CRP的调节.
- 除了是心血管风险标志物外,CRP还充当了动脉样硬化因素.
- 这些发现凸显了针对CRP相关心血管疾病中的AT1-R的治疗潜力.
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