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相关概念视频

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

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Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting
13:41

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Published on: October 17, 2011

三甲基胺N-氧化物稳定RNA的三级结构,并减弱尿素的变性效应.

Thomas C Gluick1, Sushma Yadav

  • 1Department of Chemistry and Biochemistry, P.O. Box 19065, The University of Texas at Arlington, Arlington, TX 76019, USA. tcgluick@uta.edu

Journal of the American Chemical Society
|April 10, 2003
PubMed
概括
此摘要是机器生成的。

三甲基胺N氧化物 (TMAO) 稳定RNA的三级结构,抵消尿素.

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting
13:41

Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting

Published on: October 17, 2011

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
11:49

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

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科学领域:

  • 生物化学 生化学
  • 分子生物学分子生物学
  • 结构生物学 结构生物学

背景情况:

  • 像三甲基胺N氧化物 (TMAO) 和尿素这样的奥斯莫利特可以帮助细胞在极端条件下生存.
  • 在鱼和鱼中发现的TMAO和尿素的摩尔比为1:2,其中TMAO抵消了尿素的蛋白质破坏作用.
  • 众所周知,RNA被尿素变质.

研究的目的:

  • 为了研究TMAO是否能像蛋白质一样稳定RNA的三级结构.
  • 为了确定TMAO是否对抗尿素对RNA的变性效应.

主要方法:

  • 研究了TMAO和尿素对大肠杆菌tRNAfmet的三级结构的影响.
  • 在TMAO与尿素的不同摩尔比率下检查了结构稳定性.

主要成果:

  • 发现TMAO可以稳定大肠杆菌tRNAfmet.的三级结构.
  • 在蛋白质观察到的相同比率下,TMAO有效地抵消了尿素对RNA的变性效应.
  • RNA三级结构的稳定性受溶剂成分的影响,而不仅仅是阴离子结合.

结论:

  • TMAO稳定了RNA的三级结构,并减轻了尿素的变质影响.
  • 溶剂特性在RNA三级结构稳定性中起着至关重要的作用.
  • 不利的TMAO-基骨干相互作用可能会驱动RNA折叠.