在Tribolium甲虫中,基因orthodenticle和humpback替代双体
1Interfakultäres Institut für Zellbiologie, Universität Tübingen, Abt. Genetik der Tiere, Auf der Morgenstelle 28, 72076 Tübingen, Germany. reinhard.schroeder@uni-tuebingen.de
Nature
|April 11, 2003
概括
在Tribolium甲虫中,正-1 (otd-1) 对于头部发育至关重要,与果不同. 它与背 (hb) 的相互作用对于形成头部,胸部和前腹部至关重要,这表明一种古老的模式系统.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
背景情况:
- 虫的双形态原体通过调节目标基因,如正 (otd) 和背 (hb),来控制前部模式.
- 在较高的Dipterans之外,双体同类物不存在,这使得其他昆虫的头部和胸部形成不明.
- 面粉甲虫Tribolium castaneum是一种短生殖带昆虫,为研究保存的前部模式机制提供了一个模型.
研究的目的:
- 为了研究Tribolium castaneum前面模式中保存的基因orthodenticle-1 (otd-1) 和humpback (hb) 的功能.
- 确定otd-1和hb是否在Tribolium的头部和胸部发育中发挥作用,类似于Drosophila的Bicoid.
主要方法:
- 对Tribolium otd-1信使RNA的母亲遗传分析.
- RNA干扰 (RNAi) 来减少Tribolium otd-1和hb基因表达.
- 减少otd-1和hb水平的胚胎的表型分析,包括双RNAi实验.
主要成果:
- 的otd-1信使RNA是从母亲遗传的,不同于Drosophila.
- 通过RNAi减少Tribolium otd-1导致无头胚胎,证实了它在前部模式中的作用.
- 球hb指定后面的鼻和胸部段,类似于其在Drosophila中的作用.
- 结合的otd-1/hbRNAi导致头部,胸部和前腹部发育失败,模仿强大的双突变.
结论:
- 基因orthodenticle-1 (otd-1) 对于短生殖带昆虫Tribolium castaneum的前部模式至关重要.
- 基因otd-1和hb在Tribolium中一起起作用,以指定身体前部区域,包括头部和胸部.
- 这些发现表明,otd-1和hb是昆虫中保存的古老前部模式系统的组成部分.
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