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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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相关实验视频

Updated: Jul 4, 2026

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
15:27

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

一种化染料,通过Staudinger结合激活.

George A Lemieux1, Christopher L De Graffenried, Carolyn R Bertozzi

  • 1Center for New Directions in Organic Synthesis, Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA.

Journal of the American Chemical Society
|April 17, 2003
PubMed
概括

研究人员开发了一种新的氨酸-氨酸染料,当它与化物结合时会发光. 这一进步有助于在蛋白质组学研究中分析蛋白质及其修饰.

科学领域:

  • 生物化学 生物化学
  • 化学生物学 化学生物学
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 对生物分子的特定标记对于理解细胞过程至关重要.
  • 蛋白质组学研究依赖于有效的生化和生物物理探针.
  • 现有的方法可能在具体性或易用性方面存在局限性.

研究的目的:

  • 为生物分子标签引入一种新的氨酸-氨酸染料.
  • 为了证明染料的光激活通过Staudinger与化物结合.
  • 探索其在选蛋白质和翻译后修改中的实用性.

主要方法:

  • 一种库马林-氨酸染料的合成.
  • 在染料激活过程中应用Staudinger结合反应.
  • 亚酸在细胞组件中的代谢结合.
  • 光显微镜用于检测和分析.

主要成果:

  • 氨酸-氨酸染料表现出光激活,特别是在与化物反应时.
  • 化物可以在代谢过程中被纳入细胞蛋白和寡糖.
  • 这种染料可以针对性地标记和检测亚化物修饰生物分子.

更多相关视频

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

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Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
15:27

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

结论:

  • 开发的库马林-氨酸染料是特定生物分子标签的宝贵工具.
  • 这种探测器有助于研究蛋白质及其翻译后的修改.
  • 在化学生物学和高级蛋白质组学中存在潜在的应用.