在编码序列中通过化聚胺基来抑制转录
Takanori Oyoshi1, Wakana Kawakami, Akihiko Narita
1Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental Univ., Chiyoda, Tokyo 101-0062, Japan.
Journal of the American Chemical Society
|April 17, 2003
概括
头聚胺可以化DNA,可以抑制基因转录. 这项研究表明,由毛聚胺 (ImPyPy-gamma-ImPyLDu86) 进行的特定DNA化有效地阻止了全长mRNA合成,产生了截断版本.
科学领域:
- 分子生物学分子生物学
- 化学生物学 化学生物学
- 遗传学 是一个遗传学.
背景情况:
- 基因化是修改遗传物质的一个关键机制.
- 针头聚胺是可以与特定DNA序列结合的分子.
- 了解DNA修饰剂如何影响基因表达对于治疗开发至关重要.
研究的目的:
- 为了研究DNA序列特异性化由毛聚胺对转录的影响.
- 为了确定编码区域内的化是否可以抑制基因表达.
- 评估化聚胺作为转录抑制剂的潜力.
主要方法:
- 高分辨率的聚烯胺凝电泳以分析DNA化和mRNA产物.
- 合成和使用一种特定的头针聚胺 (ImPyPy-gamma-ImPyLDu86) 和其化衍生物.
- 在体外转录测试使用含有GFP编码序列的DNA片段进行.
主要成果:
- 发针聚胺基化了993-bpDNA片段在编码和非编码链上的特定位置.
- DNA化抑制了全长mRNA的形成,并导致了截断的mRNA的产生.
- 在50nM的聚胺中观察到完全抑制全长mRNA合成.
- 一种化,非化衍生物并没有显著抑制转录.
结论:
- 化针聚胺可以通过向特定的DNA序列来有效抑制转录.
- 这种抑制甚至发生在基因编码区域内的基化位点时.
- 化聚胺是一种控制基因表达的潜在策略.
相关概念视频
Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Chromatin Structure Regulates pre-mRNA Processing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
Eukaryotic Transcription Inhibitors
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Transcription Attenuation in Prokaryotes
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Bacterial Transcription
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...


