长绳结合氧化还原中心与聚合物骨干,增强了酶"布线"水凝中的电子运输
Fei Mao1, Nicolas Mano, Adam Heller
1TheraSense Inc., 1360 South Loop Road, Alameda, California 94502, USA.
Journal of the American Chemical Society
|April 17, 2003
概括
这项研究引入了一种具有显著增强电子扩散的新型氧化还原水凝. 长绳可以有效地从酶中转移电子,从而提高葡萄糖电氧化性能.
科学领域:
- 电化学 电化学 电化学
- 聚合物科学 聚合物科学
- 生物材料是一种生物材料.
背景情况:
- 电氧化解水凝对于电化学应用至关重要.
- 提高电子扩散和酶相互作用是提高效率的关键.
- 基于的氧化还原中心提供可调节的电化学特性.
研究的目的:
- 开发一种具有增强明显电子扩散系数 (D(app)) 的氧化还原水凝.
- 为了研究绳索长度对电子传输效率的影响.
- 使用酶修饰的水凝来改善葡萄糖的电氧化.
主要方法:
- 合成具有长间距臂的交联氧化还原聚合物.
- 将三基二甲基化N,N'-二胺醇Os2+/3+) 复合物连接到聚合物骨干.
- 液凝的电化学表征,包括D ((app) 和电流密度测量.
主要成果:
- 获得了 (5.8 +/- 0.5) x 10(-6) cm(2) s(-1 的明显电子扩散系数 (D(app),比以前的水凝高出一个数量级.
- 由于长而灵活的带,证明了从葡萄糖氧化酶中有效收集电子.
- 观察到葡萄糖在 -0.36 V 与 Ag/AgCl 相比的有效电氧化,限制电流密度为 1.15 mA cm(-2) 在 -0.1 V 与 Ag/AgCl 相比.
结论:
- 通过长间距臂连接氧化还原中心显著增强水凝中的电子扩散.
- 新的Os2+/3+) 复杂和灵活的带使得高效的酶介导电催化成为可能.
- 这种先进的氧化还原水凝显示了电化学生物传感器和生物燃料电池的巨大潜力.
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