将细胞外Dpp/BMP形态基因梯度转化为反转录写梯度的转化
Bruno Müller1, Britta Hartmann, George Pyrowolakis
1Institut für Molekularbiologie, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
Cell
|April 23, 2003
概括
Dpp/BMP形态基因梯度通过控制brinker (brk) 基因表达来调节Drosophila的发育. 这项研究揭示了边缘水平如何决定基因抑制,建立精确的身体模式.
科学领域:
- 发育生物学 发展生物学
- 分子遗传学 分子遗传学
- 德洛索菲拉 (Drosophila melanogaster) 的研究研究
背景情况:
- 形态原梯度对于在胚胎发育过程中建立身体模式至关重要.
- 脱角 (Dpp) /骨形态遗传蛋白 (BMP) 途径是Drosophila发育中的一个关键信号系统.
- 布林克 (brk) 基因是DPP信号的直接转录目标.
研究的目的:
- 阐明Drosophila中对DPP/BMP形态原体的主要反应的分子机制.
- 研究brinker (brk) 基因表达梯度在模式形成中的作用.
- 确定控制基因表达的调节元素和蛋白质相互作用.
主要方法:
- 对brinker (brk) 基因调控区域的分析.
- 在Drosophila.的基因表达的基因操纵.
- 生物化学测试以确定蛋白质-DNA相互作用.
主要成果:
- 不同水平的布林克蛋白抑制不同的目标基因组.
- 该brk监管区域包含一个构成性增强剂和一个DPP监管的沉声器.
- 马德/梅迪亚和施努里蛋白质复合物直接结合到brk噪声器上.
结论:
- 通过抑制特定的基因组合,brk表达梯度对模式形成具有重要意义.
- 建立了一个分子框架,说明细胞外Dpp/BMP梯度如何导致分级的转录抑制.
- 这为通过形态原信号传递精确控制基因表达提供了洞察力.
相关概念视频
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
01:28Forced Transdifferentiation
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial transdifferentiation occurs...
Artificial transdifferentiation occurs...