灵长类动物和小鼠胚胎干细胞中的血管原生细胞的不同分化动力学
Masakatsu Sone1, Hiroshi Itoh, Jun Yamashita
1Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507 Japan.
Circulation
|April 23, 2003
概括
来自灵长类胚胎干细胞 (ESCs) 的血管原生细胞 (VPCs) 与小鼠ESCs相比表现出不同的分化动力学. 这些灵长类动物衍生的VPC对血管再生疗法具有前景.
科学领域:
- 干细胞生物学 干细胞生物学
- 血管生物学 血管生物学
- 再生医学是一种再生医学.
背景情况:
- 来自小鼠胚胎干细胞 (ES) 的血管原生细胞 (VPC) 可以分化为内皮细胞和壁细胞.
- 以前的研究确定了VEGF-R2阳性细胞作为小鼠ES细胞中的VPC.
研究的目的:
- 为了确定VPC是否存在于灵长类ES细胞中.
- 为了比较灵长类动物和小鼠ES细胞之间的VPC分化动力学.
主要方法:
- 在OP9料层上培养的未分化子ES细胞.
- 使用流细胞测量来分离VEGF-R2阳性,VE-cadherin阴性细胞.
- 在特定培养条件下,分化孤立细胞成内皮细胞和壁细胞.
- 通过使用PECAM1,ENOS,SMA和calponin等标记物评估细胞分化.
- 在3D培养中评估管道形成.
主要成果:
- 不同化的子ES细胞表达VEGF-R2,与小鼠ES细胞不同.
- 隔离的VEGF-R2+ VE-cadherin细胞分化为PECAM1+,VE-cadherin+,eNOS+内皮细胞.
- 这些细胞也分化为SMA+,calponin+壁画细胞.
- 在3D培养中,分化的细胞形成了管状结构.
- 通过VEGF治疗促进了被SMA+细胞包围的PECAM1+细胞的形成.
结论:
- 灵长类动物和小鼠ES细胞衍生的VPC分化动力学不同.
- 在灵长类动物中,VEGF-R2+ VE-cadherin-细胞作为VPCs起作用.
- 原生ES细胞研究对于血管再生的临床应用至关重要.
相关概念视频
01:56Gastrulation
52.8K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
52.8K
01:21Hematopoiesis
8.1K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
8.1K
01:19Multipotency of Hematopoietic Stem Cells
3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
01:19Mesenchymal Stem Cells
4.5K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.5K
01:10Zygotic Development And Stem Cell Formation
6.4K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
6.4K
01:27Source And Potency Of Stem Cells
5.0K
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
5.0K