隔离过的老鼠心脏对选择性β1和β2上腺体受体刺激的生物能量反应的差异
Patrick McConville1, Kenneth W Fishbein, Edward G Lakatta
1NMR Unit, Laboratory of Clinical Investigation, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Md 21224, USA.
Circulation
|April 23, 2003
概括
心脏中的β1和β2上腺素受体 (AR) 刺激会以不同的方式影响新陈代谢. 贝塔2-AR刺激导致在给定的工作负载下更大的能量负荷下降和氧气消耗,这表明不同的基板利用.
科学领域:
- 心血管生理学心血管生理学
- 代谢生物化学 代谢生物化学
- 上腺体受体信号传递
背景情况:
- 心脏中的功能差异是由β1和β2上腺素受体 (AR) 刺激引起的.
- 这些功能变异可能源于对β1-和β2-AR激活的不同代谢反应.
研究的目的:
- 在选择性β1-AR和β2-AR刺激下,研究心脏工作量和新陈代谢之间的关系.
- 为了比较β1-AR与β2-AR刺激的代谢后果,在隔离的透的老鼠心脏中.
主要方法:
- 使用隔离过的老鼠心脏接受了选择性的β1-AR和β2-AR刺激.
- 测量速度-压力产物 (RPP),含的代谢物,使用31P核磁共振 (NMR) 光谱学测量pH,通过光纤氧计测量氧气消耗 (MVO2).
- 在高恒流 (HCF) 和流量限制恒压 (CP) 条件下进行实验.
主要成果:
- 尽管与β1-AR刺激增加了较大的RPP,但对于β1-AR和β2-AR组,细胞内能量负荷的相对下降是相似的.
- 氧气消耗 (MVO2) 在HCF下与工作负载成比例增加,但在CP下类似地增加,尽管RPP不同.
- 与β2-AR刺激相比,在β1-AR刺激期间观察到较大的酸性.
结论:
- 生物能量差异表明β1-AR刺激可以访问额外的基质池,可能是通过增加糖原分解.
- 这在正常条件下通过氧化酸化促进了增强的能量生产.
- 在流量限制条件下,无氧糖解可能会在β1-AR刺激过程中促进能量产生.
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